Journal: International Journal of Molecular Sciences

Article Title: Dye-Mediated Photo-Oxidation Biomaterial Fixation: Analysis of Bioinductivity and Mechanical Properties of Bovine Pericardium for Use in Cardiac Surgery

doi: 10.3390/ijms221910768

Figure Lengend Snippet: Dye-Mediated Photo-Oxidation (DMPO)-fixed ECM Bioscaffold Promotes a Pro-Vasculogenic Paracrine Response from Human Cardiac Fibroblasts Similar to Bioactive Small Intestinal Submucosa (SIS) ECM Bioscaffold. Multiplex analysis of fibroblast growth factor-2 (FGF-2), hepatocyte growth factor (HGF), fibroblast growth factor-1 (FGF-1), vascular endothelial growth factor-A (VEGF-A), and vascular endothelial growth factor-C (VEGF-C) from conditioned media from human cardiac fibroblasts on tissue culture plastic (control; N = 7), bioactive SIS-ECM (N = 7), or DMPO-fixed ECM bioscaffold (N = 7). Values represent mean ± SEM, baseline-corrected to control. Statistical significance determined by unpaired Student’s t -test; symbols denote statistical significance (* = p -value < 0.05).

Article Snippet: Additionally, given the hypothesized bioinductivity of this material, we compared the paracrine activity of human cardiac fibroblasts seeded on DMPO-fixed bovine pericardium with those seeded on known bioactive SIS-ECM (Cor TM PATCH; CorMatrix Cardiovascular Inc., Roswell, GA, USA).

Techniques: Multiplex Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Progress in Bioengineering Strategies for Heart Regenerative Medicine

doi: 10.3390/ijms23073482

Figure Lengend Snippet: Schema of the latest biomaterials and biofabrication technologies for the generation of engineered heart muscles. 3D, three-dimensional; BP, bioprinting; EC, endothelial cell; ECM, extracellular matrix; EHT, engineered heart tissue; FRESH, freeform reversible embedding of suspended hydrogels; PDMS, polydimethylsiloxane; SWIFT, sacrificial writing into functional tissue. 1. Hydrogels: In situ gelling [ , ], Epicardial patch [ , ], Cell/biomolecule encapsulation [ , ], Casting molds [ , ]; 2. Deculllarized Bioscaffolds : ECM bioscaffolds [ , ]; 3. Microfabrication Technologies: Electronic cardiac patch , Patterning (on PDMS) , Angiochip , Anisotropic cardiac patch [ , ], Self-organizing patterned michrochamber , Electrospinning [ , ], Microfluidic chip , Muscle strip (ring shaped EHT) , Dynamic culture (cardiobundles) , Micro heart muscle arrays , Biowire TM [ , ]; 4. Organoids : Cardiac organoids [ , ]; 5. Bioprinting: Extrusion based multimaterial BP , FRESH BP [ , ], Stereolitography based BP of vasculature , Multiphoton-excited 3D BP , BP of microfibrous scaffold & ECs , SWIFT BP .

Article Snippet: Focusing on the restoration of ECM homeostasis and structure in the heart environment, other clinical trials evaluating the therapeutic effects of ECM epicardial patches (CorMatrix TM -ECM; porcine small intestinal submucosa-derived) [ , , ] or cardiac ECM hydrogels (VentriGel; decellularized porcine myocardium-derived) [ ] to the recent or late infarcted hearts have recently been completed.

Techniques: Muscles, Functional Assay, In Situ, Encapsulation, Stripping Membranes

Journal: International Journal of Molecular Sciences

Article Title: Progress in Bioengineering Strategies for Heart Regenerative Medicine

doi: 10.3390/ijms23073482

Figure Lengend Snippet: The selected in vivo preclinical studies and human clinical trials of cardiac tissue engineering-based therapies for heart regeneration and repair. CABG, coronary artery bypass grafting; CM, cardiomyocyte; EC, endothelial cell; ECM, extracellular matrix; EF, ejection fraction; EHM, engineered heart muscle; hESC, human embryonic stem cell; hiPSC, human inducible pluripotent stem cell; HF, heart failure; IGF, insulin-like growth factor; IJ, injection; IR, ischemia reperfusion injury; MI, myocardial infarction; PLGA, poly(lactic- co -glycolic acid); PNIPAAm, poly(N-isopropylacrylamide); SMC, smooth muscle cell.

Article Snippet: Focusing on the restoration of ECM homeostasis and structure in the heart environment, other clinical trials evaluating the therapeutic effects of ECM epicardial patches (CorMatrix TM -ECM; porcine small intestinal submucosa-derived) [ , , ] or cardiac ECM hydrogels (VentriGel; decellularized porcine myocardium-derived) [ ] to the recent or late infarcted hearts have recently been completed.

Techniques: In Vivo, Clinical Proteomics, Injection, Stripping Membranes, In Situ, Preserving

Journal: International Journal of Molecular Sciences

Article Title: Progress in Bioengineering Strategies for Heart Regenerative Medicine

doi: 10.3390/ijms23073482

Figure Lengend Snippet: Schema of the latest biomaterials and biofabrication technologies for the generation of engineered heart muscles. 3D, three-dimensional; BP, bioprinting; EC, endothelial cell; ECM, extracellular matrix; EHT, engineered heart tissue; FRESH, freeform reversible embedding of suspended hydrogels; PDMS, polydimethylsiloxane; SWIFT, sacrificial writing into functional tissue. 1. Hydrogels: In situ gelling [ , ], Epicardial patch [ , ], Cell/biomolecule encapsulation [ , ], Casting molds [ , ]; 2. Deculllarized Bioscaffolds : ECM bioscaffolds [ , ]; 3. Microfabrication Technologies: Electronic cardiac patch , Patterning (on PDMS) , Angiochip , Anisotropic cardiac patch [ , ], Self-organizing patterned michrochamber , Electrospinning [ , ], Microfluidic chip , Muscle strip (ring shaped EHT) , Dynamic culture (cardiobundles) , Micro heart muscle arrays , Biowire TM [ , ]; 4. Organoids : Cardiac organoids [ , ]; 5. Bioprinting: Extrusion based multimaterial BP , FRESH BP [ , ], Stereolitography based BP of vasculature , Multiphoton-excited 3D BP , BP of microfibrous scaffold & ECs , SWIFT BP .

Article Snippet: Epicardial infarct repair using CorMatrix TM -ECM (NCT02887768) , Acute~subacute MI (within 6 weeks); CABG scheduled , Phase 1 completed , Cardiac patch , Porcine small intestine-derived ECM epicardial patch , acellular , Epicardium , [ , , ] .

Techniques: Muscles, Functional Assay, In Situ, Encapsulation, Stripping Membranes

Journal: International Journal of Molecular Sciences

Article Title: Progress in Bioengineering Strategies for Heart Regenerative Medicine

doi: 10.3390/ijms23073482

Figure Lengend Snippet: The selected in vivo preclinical studies and human clinical trials of cardiac tissue engineering-based therapies for heart regeneration and repair. CABG, coronary artery bypass grafting; CM, cardiomyocyte; EC, endothelial cell; ECM, extracellular matrix; EF, ejection fraction; EHM, engineered heart muscle; hESC, human embryonic stem cell; hiPSC, human inducible pluripotent stem cell; HF, heart failure; IGF, insulin-like growth factor; IJ, injection; IR, ischemia reperfusion injury; MI, myocardial infarction; PLGA, poly(lactic- co -glycolic acid); PNIPAAm, poly(N-isopropylacrylamide); SMC, smooth muscle cell.

Article Snippet: Epicardial infarct repair using CorMatrix TM -ECM (NCT02887768) , Acute~subacute MI (within 6 weeks); CABG scheduled , Phase 1 completed , Cardiac patch , Porcine small intestine-derived ECM epicardial patch , acellular , Epicardium , [ , , ] .

Techniques: In Vivo, Clinical Proteomics, Injection, Stripping Membranes, In Situ, Preserving